Endometrial dating chart
Steroid hormone control of endometrial, epithelial, stromal, and presumably endothelial cells is mediated by estrogen receptors and progesterone receptors. They have high affinity to bind estradiol and progesterone, respectively.These steroid receptors are specific proteins concentrated exclusively in the nuclei of both endometrial epithelial and stromal cells, as well as the endothelial cells of stromal capillaries. This chapter contains a review of the technical procedures for handling endometrial tissues and a discussion of the morphologic aspects of the endometrium, focusing on the interpretation and understanding of the physiomorphology of the endometrial cycle.
After examining the specimen, the pathologist should attempt to correlate histology with clinical history.
In cases of clinical membranous dysmenorrhea, the endometrial biopsy should be taken on cycle days 5–10.
In these instances, the histologic specimens contain large fragments (casts) of endometrium, often with focal Arias-Stella reaction, or star-shaped glands with dense stroma alternating with foci of normal menstrual endometrium.
In cases in which little or no tissue is obtained but the endometrium was penetrated with the aspirator, a repeat procedure should be performed.
If the repeat aspiration still yields little tissue, one can assume severe endometrial atrophy or obstructing endometrial polyp.Method To ensure a good specimen for morphologic interpretation, a biopsy sample should be taken from both the anterior and the posterior endometrium and fixed immediately in 10% buffered formalin.